| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Coxiella burnetii DNA, Qualitative Real-Time PCR
Test Code16596X
CPT Codes
87798
Preferred Specimen
0.7 serum, CSF or bronchial alveolar lavage (BAL)/ wash in a sterile screw cap container • 3 mL respiratory swab in M4 VCM medium (green-cap) tube or equivalent
Minimum Volume
Respiratory swab: 3 mL
CSF, bronchial alveolar lavage (BAL)/wash: 0.3 mL
CSF, bronchial alveolar lavage (BAL)/wash: 0.3 mL
Other Acceptable Specimens
Whole blood collected in a lavender-top (EDTA) or yellow-top (ACD) tube or tissue collected in a sterile container
Instructions
Tissue should be frozen on dry ice and transported frozen via overnight express. All other specimens should be transported refrigerated (2-8°C) via overnight express.
Transport Temperature
Serum, CSF, bronchial lavage (BAL), whole Blood: Refrigerated (cold packs)
Tissue: Frozen
Tissue: Frozen
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Refrigerated: 7 days
Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days
Clinical Significance
Real-Time PCR detection of Coxiella burnetii can be used as a tool in the diagnosis of both acute and chronic Q fever. Real-Time PCR assays offer superior sensitivity, rapid test result turn-around, and the ability to detect C. burnetii DNA in multiple specimen types.
