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Cortisol, Free, LC/MS/MS, 24-Hour Urine
Test Code11280X
CPT Codes
82530
Includes
CPT code 81050 may be added at an additional charge for volume measurement
Preferred Specimen
2 mL urine from a 24-hour collection (no preservatives)
Minimum Volume
0.5 mL
Other Acceptable Specimens
25 mL 6N HCl • 10 mL concentrated glacial acetic acid • 10 g boric acid
Instructions
Record 24-hour urine volume on test requisition and urine vial. Reference ranges do not apply to random urine samples.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 14 days
Refrigerated: 21 days
Frozen: 90 days
Refrigerated: 21 days
Frozen: 90 days
Methodology
Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Setup Schedule
Night Sets up 6 days a week.
Clinical Significance
Urinary Free Cortisol is useful in the detection of patients with Cushing's syndrome for whom Free Cortisol concentrations are elevated.