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Cortisol, Free and Total, LC/MS
Test Code37077
CPT Codes
82530, 82533
Preferred Specimen
2 mL serum collected in a red-top tube (no gel) and poured off into two separate transport tubes containing 1 mL in each tube
Minimum Volume
0.5 mL in each tube
Other Acceptable Specimens
Plasma collected in an EDTA (lavender-top) tube and poured off into two separate transport tubes containing 1 mL in each tube
Instructions
Collect 2 mL (1 mL minimum) serum or plasma and aliquot into two separate transport tubes, with 1 mL (0.5 mL minimum) in each transport tube.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 1 year
Refrigerated: 7 days
Frozen: 1 year
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Received room temperature • Serum separator tube (SST)
Methodology
Equilibrium Dialysis • Chromatography/Mass Spectrometry
Setup Schedule
Set up: Mon-Fri; Report available: 2-6 days
Clinical Significance
Free cortisol is useful in the detection of patients with Cushing's syndrome for whom free cortisol concentrations are elevated.
