Cortisol Response to ACTH Stimulation, Serum

Test Code

38149

CPT Codes
82533 (x4)

Includes

Cortisol Response, Baseline
Cortisol Response, 15 Minutes
Cortisol Response, 30 Minutes
Cortisol Response, 60 Minutes

Preferred Specimen

1 mL serum collected in each of four separate red top tubes (no gel)

Minimum Volume

0.2 mL for each timed specimen

Other Acceptable Specimens

Plasma collected in: EDTA (lavender-top) tube

Instructions

For each timed specimen submitted, each tube must be clearly marked with time drawn. Submit all tubes with one test request form.

Draw morning baseline specimen. Administer 250 mcg Cortrosyn. Draw additional specimens at 15, 30 and 60 minutes.

Note: Cortrosyn (cosyntropin) not supplied by the laboratory. This test cannot be collected at a Patient Service Center.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 14 days
Refrigerated: 21 days
Frozen: 1 year

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Serum Separator Tubes (SST®)

Methodology
Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Night Sets up 6 days a week.

Clinical Significance

The cortisol response to infusion of synthetic ACTH is useful in diagnosing patients with adrenal insufficiency (Addison's disease).

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.