CD35, IHC without Interpretation

Test Code

40041X

CPT Codes
88341

Preferred Specimen

Formalin fixed paraffin embedded tissue in IHC specimen transport kit

Other Acceptable Specimens

4-micron unstained slides (x5) • Fixed tissue

Instructions

Tumor paraffin block (formalin-fixed only) or five 4-micron unstained sections on Poly-L-Lysine or Silane coated slides. See Specimen Collection Section, Oncology. Ship at room temperature. Do not place paper labels with adhesive backing on slides. Use pencil or xylene resistant pen to write on the frosted end of the slide only. Pathology report is required.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: Indefinite
Refrigerated: Indefinite
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Tissue in formalin

Methodology
Immunohistochemistry (IHC)

Setup Schedule

A.M.

Clinical Significance

The CD35 antigen, also known as CR1 or C3b/C4b R, is a
transmembrane protein of 160 to 250 kD which binds
complement components C3b and C4b. It mediates phogocytosis
by neutrophils and monocytes of particles coated with C3b
or C4b. CD35 antigen has an inhibitory effect on complement
activation by both the classical and alternative pathoways.
CD35 antigen is reported to be found on erythrocytes, B
cells, a subset of T cells, monocytes, macrophages cultured
in vitro, neutrophils, eosinophils, glomerular podocytes
and follicular dendritic cells. Decreased levels of CD35
antigen has been reported on B cells in patients with HIV
infection.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.