CAH Panel 4 (17-Hydroxylase Deficiency in Females)

Test Code

15274

CPT Codes
84144, 83498, 82528, 82533, 82088, 82670

Includes

Aldosterone, LC/MS/MS
Corticosterone
Cortisol, Total, LC/MS
Estradiol, Ultrasensitive, LC/MS
7-Hydroxyprogesterone
Progesterone, LC/MS

Preferred Specimen

3 mL serum collected in a red-top tube (no gel)

Patient Preparation
An early morning specimen is preferred

Minimum Volume

1.2 mL

Instructions

Specify time of day specimen was collected, patient age and sex on test request form.
Collect blood in a Vacutainer with no additives. Allow blood to clot (10-15 minutes) at room temperature. Centrifuge and separate the serum from the cells.

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 24 hours
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Serum Separator Tube (SST^®) • Moderate to gross hemolysis • Received room temperature • Grossly icteric • Glass tubes

Methodology
Extraction • Immunoassay (IA) • Chromatography/Mass Spectrometry

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

See individual assays

Clinical Significance

All steroids requiring the action of 17-Hydroxylase enzyme are low, including 17-OH Progesterone and Cortisol. Levels of estradiol and aldosterone are also low. Additional testing may be required to establish a diagnosis.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.