A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
Beta-2-Glycoprotein I Antibody (IgA)
Test Code36552
CPT Codes
86146
Preferred Specimen
1 mL plasma collected in a 3.2% sodium citrate (light blue-top) tube
Minimum Volume
0.5 mL
Other Acceptable Specimens
Serum
Instructions
Plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 5 days
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic
Methodology
Immunoassay (IA)
Setup Schedule
A.M.
Limitations
Factor VIII inhibitors may cause a false-positive lupus anticoagulation test. The temporary presence of antiphospholipid antibodies may accompany infections or certain drugs.
Clinical Significance
Beta-2-Glycoprotein 1, apolipoprotein H, is a cofactor in antiphospholipid antibody binding and is the critical antigen in the antiphospholipid antibody syndrome. Beta-2- Glycoprotein 1 antibody is more specific than cardiolipin antibody that may express reactivity in patients with syphilis and other infectious diseases.