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Anaplasma phagocytophilum DNA, Qualitative Real-Time PCR
Test Code17320
CPT Codes
87468
Preferred Specimen
0.7 mL whole blood collected in an EDTA (lavender-top) or ACD (yellow-top) tube
Minimum Volume
0.3 mL
Other Acceptable Specimens
1 tick in 70% ethanol submitted in a sterile leak-proof container
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Tick
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Tick
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Heparin whole blood
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: 1-3 days
Clinical Significance
Human granulocytic ehrlichiosis (HGE) DNA PCR is a highly sensitive and specific method to detect the agent responsible for HGE, Ehrlichia equi and Ehrlichia phagocytophilia. This assay does not recognize sequences for the human ehrlichial pathogen Ehrlichia chaffeensis. The diagnosis of HGE infection should be considered in conjunction with clinical presentation and additional established clinical tests. A negative PCR result indicates the absence of HGE at detectable levels in the sample tested and does not exclude the diagnosis of disease.
