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Salmonella/Shigella Cult, Campy EIA and Shiga Toxin reflex E.coli O157 Cult
Test Code32114
CPT Codes
87045, 87427, 87449
Includes
Shiga Toxins, EIA with Reflex to E. coli O157, Culture
Salmonella and Shigella, Culture
Campylobacter species Antigen, EIA
Shiga Toxins, EIA with Reflex to E. coli O157, Culture: If Shiga Toxin, EIA is Detected, then Escherichia coli O157, Culture will be performed at an additional charge (CPT code(s): 87046).
Salmonella and Shigella, Culture: If culture is positive, an identification will be performed at an additional charge (CPT code(s): 87077).
Antibiotic susceptibilities are only performed when appropriate (CPT code(s): 87186).
Salmonella and Shigella, Culture
Campylobacter species Antigen, EIA
Shiga Toxins, EIA with Reflex to E. coli O157, Culture: If Shiga Toxin, EIA is Detected, then Escherichia coli O157, Culture will be performed at an additional charge (CPT code(s): 87046).
Salmonella and Shigella, Culture: If culture is positive, an identification will be performed at an additional charge (CPT code(s): 87077).
Antibiotic susceptibilities are only performed when appropriate (CPT code(s): 87186).
Preferred Specimen
10 grams stool submitted in Cary-Blair stool culture transport medium
Minimum Volume
5 grams
Instructions
Collect in clean, dry container and then transfer a minimum of 1 gram or 1 mL into a Cary-Blair stool culture transport medium. Fluid level should reach line on vial. Infectious substance - See Infectious Substances in the Transporting Specimens to Quest Diagnostics section of Specimen Collection and Handling.
Transport Temperature
Room temperature
Specimen Stability
Stool or swab in Cary-Blair
Room temperature: 4 days
Refrigerated: 4 days
Frozen: Unacceptable
Room temperature: 4 days
Refrigerated: 4 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unpreserved specimen • Specimen in diaper • Stool specimens in medium other than those listed • Stool specimens that have been previously concentrated • swab specimens
Methodology
Bacterial Culture • Aerobic Isolation and Identification Procedures • Immunoassay with Culture Reflex • Immunoassay (IA)
Setup Schedule
Sets up 7 days a week.
Report Available
Reports in 3 to 6 days.
Clinical Significance
Salmonella, Shigella, and Campylobacter are considered gastro-intestinal pathogens. Identification of these organisms is important for treatment and infection control. The Shiga toxin assay detects toxins produced by enterohemorrhagic E. coli and other enteric organisms which have been isolated from patients who have hemorrhagic colitis with or without hemolytic-uremic syndrome (HUS).
