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QuestAssureD™25-OH Vitamin D (Total), LC/MS/MS
Test Code91841
CPT Codes
82306<br /> **Restricted Use ** This is a limited access test code and is available only to Quest Business Units<br />
Preferred Specimen
0.5 mL serum
Patient Preparation
Fasting preferred, but not required
Minimum Volume
0.3 mL
Instructions
Collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a serum separator tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.
Alternatively, collect blood in a serum separator tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 21 days
Refrigerated: 21 days
Frozen: 21 days
Refrigerated: 21 days
Frozen: 21 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Gross lipemia • Heparinized or EDTA Plasma • Sample out of stability • Icteric samples • Serum not separated from SST gel or clot w/in 48hr
Methodology
Chromatography/Mass Spectrometry
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report Available: 3-4 days
Clinical Significance
Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25OH D3 - the endogenous form of the vitamin and 25OH D2 - the analog form used to treat 25OH Vitamin D3 deficiency.
