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Mycobacteria, Culture, with Fluorochrome Smear
Test Code4554CS
CPT Codes
87015, 87116, 87206, 87118 (x2)<br /> *** RESTRICTED USE *** This code is available for St. Joseph Health ONLY. <br />
Preferred Specimen
10 mL body fluids or lower respiratory tract specimens or
10 mL gastric lavage or
2 mL CSF collected in a sterile, leak-proof container or
2 grams fresh (unfixed) tissue or
40 mL urine collected in a sterile, leak-proof container or
1 mL exudate, aspirate, lesion material, ocular specimen collected in a sterile, leak-proof container
10 mL gastric lavage or
2 mL CSF collected in a sterile, leak-proof container or
2 grams fresh (unfixed) tissue or
40 mL urine collected in a sterile, leak-proof container or
1 mL exudate, aspirate, lesion material, ocular specimen collected in a sterile, leak-proof container
Minimum Volume
2 mL gastric lavage, body fluids or lower respiratory tract specimens
1 mL CSF
1 gram fresh (unfixed) tissue or
20 mL urine
1 mL exudate, aspirate, lesion material, ocular specimen
1 mL CSF
1 gram fresh (unfixed) tissue or
20 mL urine
1 mL exudate, aspirate, lesion material, ocular specimen
Other Acceptable Specimens
Swabs in Amies liquid, Amies gel swab transport system, Stuarts or ESwab, ONLY if tissue biopsy or wound aspirate is not available; Samples in Anaerobic Transport Medium, ONLY if it is an irreplaceable sample type; Throat/oral/sputum swabs from cystic-fibrosis patients
Instructions
Tissue and biopsy material: Sterile, leak-proof container with small amount of saline, no fixative or preservative
Gastric lavage fluid: Sterile, leak-proof container. Must be neutralized with sodium bicarbonate within 4 hours of collection.
See the alphabetical listing in the Microbiology section of Specimen Collection and Handling for further instructions.
Gastric lavage fluid: Sterile, leak-proof container. Must be neutralized with sodium bicarbonate within 4 hours of collection.
See the alphabetical listing in the Microbiology section of Specimen Collection and Handling for further instructions.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 5 days
Frozen: Unacceptable
Refrigerated: 5 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
24 hr. pooled urine or sputum • Dry swabs • Specimens broken in transit • Specimens received in alcohol • Formalin • EDTA • Lithium Heparin • Conventional blood culture bottles • Specimens received in expired transports • Specimens that leaked during transit • Throat/oral/sputum swabs from non-cystic-fibrosis patients • Urine specimens in preservative tubes
Methodology
Continuous Monitoring Broth Culture System & Conventional Culture • Microscopic Exam with Fluorochrome Stain
Setup Schedule
Sets up 7 days a week.
Clinical Significance
There are more than 70 species of mycobacteria of which the primary pathogen is M. tuberculosis. Tuberculosis is a chronic, granulomatous disease caused by M. tuberculosis. Identification, and, if appropriate, antibiotic sensitivity, is required to initiate necessary therapy.
