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Lipoprotein Fractionation, NMR
Test Code37847
CPT Codes
83704
Includes
LDL P, Small LDL P, LDL Size
HDL P, Large HDL P, HDL Size
Large VLDL P, VLDL Size
HDL P, Large HDL P, HDL Size
Large VLDL P, VLDL Size
Preferred Specimen
2 mL serum collected in a red-top tube (no gel)
Patient Preparation
Patient should be fasting 12 hours
Minimum Volume
1 mL
Instructions
1. Gently invert draw tube 5X (DO NOT SHAKE).
2. Let blood clot for 30-60 minutes at room temperature.
3. Centrifuge for 10 minutes.
4. Aliquot top layer from red-top draw tube and place SERUM in transport tube and dispose of draw tube. Label the transport tube as "No Additive Serum Red". ONLY ship No Additive Serum in transport tube.
5. Store and transport refrigerated. It is acceptable to store frozen and ship refrigerated. Do not ship frozen on dry ice.
2. Let blood clot for 30-60 minutes at room temperature.
3. Centrifuge for 10 minutes.
4. Aliquot top layer from red-top draw tube and place SERUM in transport tube and dispose of draw tube. Label the transport tube as "No Additive Serum Red". ONLY ship No Additive Serum in transport tube.
5. Store and transport refrigerated. It is acceptable to store frozen and ship refrigerated. Do not ship frozen on dry ice.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen -20° C: 10 days
Frozen -70° C: 28 days
Refrigerated: 7 days
Frozen -20° C: 10 days
Frozen -70° C: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Serum separator tube (SST)
Methodology
Nuclear Magnetic Resonance
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics Cardiometabolic Center of Excellence at Cleveland HeartLab. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Sets up 7 days a week.
Clinical Significance
The Lipoprotein Fractionation, NMR test is used to help assess the risk for cardiovascular disease (CVD) in patients with intermediate or high risk based on traditional or emerging risk factors, and to assess therapeutic response in patients undergoing lipid-lowering therapy.
