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Protein S Activity with Reflex to Protein S Antigen, Total and Free
Test Code17494X
CPT Codes
85306
Includes
If Protein S Activity is <60% for females or <70% for males, then Protein S Antigen Total and Free will be performed at an additional charge (CPT code(s): 85305, 85306).
Preferred Specimen
2 mL plasma collected in 3.2% sodium citrate (light. blue-top) tube
Minimum Volume
1 mL
Instructions
Draw blood in 3.2% sodium citrate ( light blue-top) tubes, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collection. Using a plastic pipett, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into new plastic screw-cap vial(s). Freeze immediately and transport on dry ice.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Refrigerated: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis
Methodology
Clot Detection • Immunotrubidimetric
Setup Schedule
See individual assays