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MGMT(O6-Methylguanine-DNA Methyltransferase) Gene Methylation Assay
Test Code13637CS
CPT Codes
81287<br><strong>This test is not available for New York patient testing.</strong><br>*** Restricted Use. This code is available to Client #55001, 51613, 53880,54656, 58536 and All Kaiser ONLY ***
Preferred Specimen
Formalin-fixed, paraffin-embedded (FFPE) tissue blocks or 4 (2 minimum) unstained slides at 5 µM and one matching H&E slide or 5 (3 minimum) precut unstained slides from a paraffin block in 5 µM sections.
(Note: Samples with >4 mm2 and > or = 50% tumor content are preferred.)
(Note: Samples with >4 mm2 and > or = 50% tumor content are preferred.)
Instructions
Please send four precut unstained slides from formalin fixed paraffin-embedded tissue block in 5 mm sections or a formalin fixed paraffin-embedded tissue block. Mininum volume: 2 unstained slides at 5 mm.
Please provide a copy of the pathology report. Sample submission without a copy of the pathology report may delay testing.
(Note: Samples with >4 'square millimeter' and > or equals to 50% tumor content are preferred>)
Please provide a copy of the pathology report. Sample submission without a copy of the pathology report may delay testing.
(Note: Samples with >4 'square millimeter' and > or equals to 50% tumor content are preferred>)
Transport Temperature
Room temperature
Specimen Stability
Room temperature: Indefinite
Refrigerated: Unacceptable
Frozen: Unacceptable
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Tumor block containing insufficient tumor tissue • Tumor fixed in a heavy metal fixative • Broken or stained slides
Methodology
Quantitative methylation-specific polymerase chain reaction (PCR)
Setup Schedule
Sets up 5 days a week.
Clinical Significance
MGMT (O6-methylguanine-DNA methyltransferase) is a DNA
repair enzyme that is involved in the repair of damage
caused by a variety of DNA cross-linking compounds,
including alkylating agents. Increased methylation of the
MGMT gene promoter region causes dimished or silenced
expression of the gene, making cells more sensitive to DNA
damage. The relationship has been shown for glioblastomas
and alkylating agents such as temozolomide (Temodar(R)).
repair enzyme that is involved in the repair of damage
caused by a variety of DNA cross-linking compounds,
including alkylating agents. Increased methylation of the
MGMT gene promoter region causes dimished or silenced
expression of the gene, making cells more sensitive to DNA
damage. The relationship has been shown for glioblastomas
and alkylating agents such as temozolomide (Temodar(R)).
