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Glucose Tolerance Test, 5 Specimens
Test Code10560
CPT Codes
82951, 82952 (x2)<br /> **Restricted use** This code is available for client 55419 only.
Preferred Specimen
1 mL plasma collected in each of 5 separate fluoride/oxalate (gray-top) tubes
Patient Preparation
Fasting required. Fasting is defined as no consumption of food or beverage other than water for at least 8 hours before testing.
Minimum Volume
0.5 mL [x5]
Other Acceptable Specimens
Plasma collected in each of 5 separate lithium or sodium heparin (green-top) tubes • Serum collected in each of 5 tubes
Instructions
Immediately after a fasting specimen is obtained, have patient ingest 75 g load of glucose. Draw subsequent specimens per physician's order. If not specified by the physician, draw specimens 1, 2, 3 and 4 hours later. Label each with the specific draw time.
Note: Serum and plasma submissions must be separated from cells. Fasting required. Fasting is defined as no consumption of food or beverage other than water for at least 8 hours before testing.
Note: Serum and plasma submissions must be separated from cells. Fasting required. Fasting is defined as no consumption of food or beverage other than water for at least 8 hours before testing.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Plasma (fluoride/oxalate)
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Plasma (Heparin)
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 28 days
Serum
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Plasma (Heparin)
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 28 days
Serum
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unspun serum separator tube • Red-top or non-plasma separator (PST) green-top tubes • Anticoagulants other than lithium heparin, sodium heparin or fluoride/oxalate
Methodology
Spectrophotometry
Setup Schedule
Sets up 6 days a week.
Report Available
Reports in 1 day.
Clinical Significance
For appropriate interpretation of this test, the patient must fast overnight and ingest a 75 g load of glucose, immediately after a fasting specimen is obtained (for children, the glucose load is 1.75 g/Kg of body weight, up to 75). The diagnosis of diabetes is made if glucose exceeds 200 mg/dL in the 2 hour specimen and in any preceding specimen.
