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Streptococcus Group B DNA, PCR with Broth Enrichment
Test Code91768
CPT Codes
87081, 87150
Preferred Specimen
Vaginal/rectal swab in Amies gel (blue-cap) transport medium or Amies liquid (red-cap) medium or Eswab
Other Acceptable Specimens
Vaginal/rectal swab in Liquid Stuart transport medium
Instructions
In order to obtain an adequate specimen, the procedure for specimen collection must be followed closely. Using recommended swab, vaginal-rectal specimens are collected according to the following procedure:
1. Wipe away excessive amount of secretion or discharge from the vaginal area.
2. Carefully insert the swab into the lower one-third part of vagina, and sample secretions from the mucosa.
3. Carefully insert the same swab, approximately 2.5 cm beyond the anal sphincter, and gently rotate to sample anal crypts.
4. Replace the swab in its container.
Note: Endocervical, perianal, perineal and perirectal are not to be used for this test, and a speculum should not be used for culture collection.
1. Wipe away excessive amount of secretion or discharge from the vaginal area.
2. Carefully insert the swab into the lower one-third part of vagina, and sample secretions from the mucosa.
3. Carefully insert the same swab, approximately 2.5 cm beyond the anal sphincter, and gently rotate to sample anal crypts.
4. Replace the swab in its container.
Note: Endocervical, perianal, perineal and perirectal are not to be used for this test, and a speculum should not be used for culture collection.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 24 hours
Refrigerated: 6 days
Frozen: Unacceptable
Refrigerated: 6 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen • Specimens in transport media other than those listed • LIM broth received room temperature
Methodology
Culture Enhanced • Polymerase Chain Reaction (PCR)
Setup Schedule
Set up: Daily; Report available: 2-3 days
Clinical Significance
Detect Group B Streptococcus (GBS) vaginal/rectal colonization to prevent GBS sepsis and other GBS infections in newborns.
