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Parainfluenza Virus (Types 1 to 4) RNA, Qualitative Real-Time PCR
Test Code91228
CPT Codes
87631
Preferred Specimen
Nasopharyngeal or oropharyngeal swab submitted in 3 mL M4 media or VCM medium (green-cap) tube, or UTM equivalent or
2 mL bronchial lavage or sputum collected in a plastic sterile leak-proof container
2 mL bronchial lavage or sputum collected in a plastic sterile leak-proof container
Minimum Volume
0.6 mL
Instructions
Respiratory Samples in M4 media: Use sterile vials containing 3 mL of sterile M4 media. If using swabs, use only sterile Dacron or Rayon swabs. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator stick off near the tip to permit tightening of the cap.
Sputum: Collect in sputim collection kit or a sterile, plastic container with a leak-proof cap. Bronchial
Lavage/wash: Collect in a sterile container with a leak-proof cap.
Nasopharyngeal aspirate/wash: Collect in a sterile container with a leak-proof cap.
Tracheal lavage/wash: Collect in a sterile container with a leak-proof cap.
Sputum: Collect in sputim collection kit or a sterile, plastic container with a leak-proof cap. Bronchial
Lavage/wash: Collect in a sterile container with a leak-proof cap.
Nasopharyngeal aspirate/wash: Collect in a sterile container with a leak-proof cap.
Tracheal lavage/wash: Collect in a sterile container with a leak-proof cap.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Swabs
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Bronchial lavage/wash
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days
Sputum
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days
Methodology
Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report Available: 1-3 days
Clinical Significance
Human parainfluenza virus (types 1, 2, 3 and 4) are important respiratory pathogens and are major causes of upper and lower respiratory tract diseases. A multiplex Real-Time RT-PCR assay offers the advantages of increased specificity and sensitivity, identification of HPIV type in a single reaction, and rapid availability of the results. All of these factors will assist the clinician in the diagnosis of respiratory disease, decrease inappropriate use of antibiotics, as well as reduce time of hospitalization and help prevent nosocomial infections.
