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Human Metapneumovirus RNA, Qualitative, Real-Time PCR
Test Code40034
CPT Codes
87798
Preferred Specimen
Nasopharyngeal or oropharyngeal swab submitted in 3 mL M4 media or VCM medium (green-cap) tube, or UTM equivalent or
2 mL bronchial lavage collected in a plastic sterile leak-proof container
2 mL bronchial lavage collected in a plastic sterile leak-proof container
Minimum Volume
0.6 mL
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Swabs
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days
Bronchial lavage
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 30 days
Room temperature: 7 days
Refrigerated: 14 days
Frozen: 30 days
Bronchial lavage
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Sputum
Methodology
Real-Time Polymerase Chain Reaction (PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days
Clinical Significance
Human metapneumovirus is a recently identified human pathogen that is becoming widely recognized as a cause of respiratory infection, particularly in children. Symptoms range from asymptomatic infection to severe bronchitis and bronchiolitis; infection is clinically indistinguishable from RSV (respiratory syncytial virus). The detection of human metapneumovirus (hMPV) RNA is based upon reverse transcription of specific conserved hMPV genomic RNA sequences followed by real-time PCR amplification.
