Bacterial 16S rDNA Sequencing

Test Code

17221

CPT Codes
87153

Preferred Specimen

Culture isolate in agar slant double-walled container

Instructions

Preferred specimen is culture isolate in an agar slant in a double walled container. Mixed cultures will not be accepted. Subculture isolate to the appropriate slant and submit in double walled container.

Transport Temperature

Room temperature

Specimen Stability

Room temperature: Determined by viability
Refrigerated: Determined by viability
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Mixed culture • Received frozen

Methodology
16S Sequencing

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

P.M. Sets up 3 days a week.

Report Available

Reports in 9 to 12 days.

Clinical Significance

Bacterial 16S rDNA sequencing is a genotypic test used as an adjunct to the phenotypic characteristics of an unknown bacterial isolate in order to determine the genus and/or the genus and species of the isolate. Genotypic tests are useful in the identification of unusual and new emerging pathogens.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.