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Chlamydophila pneumoniae, DNA, Qualitative, Real-Time PCR
Test Code16003X
CPT Codes
87486
Preferred Specimen
Throat or nasopharyngeal swab in 3 mL M4 or V-C-M/UTM
Minimum Volume
0.3 mL
Other Acceptable Specimens
1 mL bronchial lavage/wash or sputum in a sterile leak-proof container
Instructions
Respiratory Samples in M4 Media: Use sterile vials containing 3 mL of sterile M4 media. If using swabs, use only sterile Dacron or rayon swabs or rayon swabs. Do NOT use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator sticks off near the tip to permit tightening of the cap.
Sputum: Collect in a sputum collection kit or a sterile, plastic container with a leak-proof cap.
Bronchial Lavage: Collect in a sterile container with a leak-proof cap.
Sputum: Collect in a sputum collection kit or a sterile, plastic container with a leak-proof cap.
Bronchial Lavage: Collect in a sterile container with a leak-proof cap.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 14 days
Frozen: 30 days
Refrigerated: 14 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimen containing heparin • Specimen with calcium alginate swabs
Methodology
Real-Time Polymerase Chain Reaction (PCR)
Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days
Clinical Significance
This test is used to determine the presence of Chlamydophila pneumoniae in a patient's specimen. Organisms may be detected by PCR prior to detection by immunological methods. PCR provides more rapid results than other methods, including culture.
