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Protein, Total and Protein Electrophoresis, 24 Hour Urine and Immunofixation
Test Code38944
CPT Codes
82570, 84156, 84166, 86335
Includes
Protein, Total, 24-Hour Urine with Creatinine
Protein Electrophoresis, 24-Hour Urine (UPEP)
Immunofixation (IFE), Urine
CPT code 81050 may be added at an additional charge for volume measurement
Protein Electrophoresis, 24-Hour Urine (UPEP)
Immunofixation (IFE), Urine
CPT code 81050 may be added at an additional charge for volume measurement
Preferred Specimen
25 mL 24-hour urine collected in an unpreserved, plastic urine container
Minimum Volume
15 mL
Instructions
Record total volume on test requisition and specimen container
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 4 days
Refrigerated: 5 days
Frozen: 30 days
Refrigerated: 5 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Acidified urine
Methodology
Electrophoresis • Immunofixation • Spectrophotometry (SP)
Setup Schedule
Night Sets up 6 days a week.
Clinical Significance
Protein electrophoresis evaluates the major protein fractions found in urine. Normally, only small amounts of albumin are seen. Urinary protein electrophoresis is useful in classifying the type of renal damage, if present. Immunofixation is useful in characterizing M-components observed in the protein electrophoresis and in identifying light-chain disease.