| A B C D E F G H I J K L M N O P Q R S T U V W X Y Z # |
QuestAssureD™ for Infants, 25-Hydroxyvitamin D
Test Code91935
CPT Codes
82306
Includes
Vitamin D, 25-OH, Total
Vitamin D, 25-OH, D3
Vitamin D, 25-OH, D2
Vitamin D, 25-OH, D3
Vitamin D, 25-OH, D2
Preferred Specimen
0.5 mL serum
Patient Preparation
Fasting specimens are preferred but not required
Minimum Volume
0.2 mL
Instructions
Collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a Serum Separator Tube (SST®) at room temperature, centrifuge and remove from the gel within 48 hours.
Alternatively, collect blood in a Serum Separator Tube (SST®) at room temperature, centrifuge and remove from the gel within 48 hours.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Plasma • Serum not separated from Serum Separator Tube (SST®) • Serum not separated from gel or clot within 48 hours • Grossly icteric
Methodology
Chromatography/Mass Spectrometry
Setup Schedule
Sets up 7 days a week.
Report Available
Reports in 2 days.
Clinical Significance
While measurements of 25(OH)D provide a good index of circulating Vitamin D activity in patients not suffering from renal disease, for infants less than 3 years of age it is important to remove circulating levels of the inactive c3-epimer of Vitamin D from the sample so that total Vitamin D levels are not falsely elevated. Falsely elevated results could erroneously make the child appear to be within normal limits and result in potential under-treatment. Levels of c3-epimer in older children and adults are usually negligible and do not cause falsely elevated Vitamin D results.
This assay employs advanced liquid chromatography - tandem mass spectrometry, which enables the chromatographic separation of the c3-epimer from 25-OH Vitamin D, thereby allowing accurate measurement in the presence of c3-epimer. While this assay will produce accurate Vitamin D results on patients of any age, it is specifically indicated for infants less than 3 years of age.
This assay employs advanced liquid chromatography - tandem mass spectrometry, which enables the chromatographic separation of the c3-epimer from 25-OH Vitamin D, thereby allowing accurate measurement in the presence of c3-epimer. While this assay will produce accurate Vitamin D results on patients of any age, it is specifically indicated for infants less than 3 years of age.
