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Cardio IQ® Vitamin D, 25-Hydroxy
Test Code91735
CPT Codes
82306
Includes
Vitamin D, 25-OH, Total
Vitamin D, 25-OH, D3
Vitamin D, 25-OH, D2
Vitamin D, 25-OH, D3
Vitamin D, 25-OH, D2
Preferred Specimen
0.5 mL serum
Patient Preparation
Fasting preferred, but not required
Minimum Volume
0.2 mL
Instructions
Minimize sample exposure to direct sunlight.
SST RF -
1. Gently invert 5X (Do not shake!).
2. Let blood clot for 30 min. at room temperature.
3. Centrifuge for 10 min.
4. Store and transport RF.
SST RF -
1. Gently invert 5X (Do not shake!).
2. Let blood clot for 30 min. at room temperature.
3. Centrifuge for 10 min.
4. Store and transport RF.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 21 days
Refrigerated: 21 days
Frozen: 185 days
Refrigerated: 21 days
Frozen: 185 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens other than serum • Improper labeling • Samples not stored properly • Samples older than stability limits • Gross hemolysis
Methodology
Chromatography/Mass Spectrometry
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics Cardiometabolic Center of Excellence at Cleveland HeartLab. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Night Sets up 7 days a week.
Clinical Significance
Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25OH D3 - the endogenous form of the vitamin and 25OH D2 - the analog form used to treat 25OH Vitamin D3 deficiency.