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Progesterone, Immunoassay
Test Code745
CPT Codes
84144
Preferred Specimen
1 mL serum
Minimum Volume
0.5 mL
Instructions
DHEA supplementation causes a false elevation of values in this standard immunoassay method for progesterone. Physicians using progesterone levels as a criterion for triggering ovulation in an IVF cycle in patients supplemented with DHEA should assess progesterone levels using a LC/MSMS Progesterone assay, such as order code 17183 performed at Quest Diagnostics San Juan Capistrano.
NOTE: Do not order progesterone testing as an add on to a barrier gel specimen older than 72 hours. Progesterone binds to barrier gel causing decreased values.
NOTE: Do not order progesterone testing as an add on to a barrier gel specimen older than 72 hours. Progesterone binds to barrier gel causing decreased values.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 72 hours
Refrigerated: 7 days
Frozen: 28 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Serum stored on barrier gel for more than 72 hours
Methodology
Immunoassay (IA)
Setup Schedule
Set up: Mon-Sat; Report available: Next day
Limitations
Heterophil antibodies may produce falsely-elevated results.
Clinical Significance
Levels increase sharply during the luteal phase of the menstrual cycle. The level increases from 9 to 32 weeks of pregnancy.
