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Vitamin B2 (Riboflavin)
Test Code36399
CPT Codes
84252
Preferred Specimen
1 mL frozen serum collected in a red-top tube (no-gel) - protected from light
Patient Preparation
Overnight fasting is required. Avoidance of vitamin supplements for 24 hours prior to collection is preferred.
Minimum Volume
0.5 mL
Other Acceptable Specimens
Frozen plasma collected in: EDTA (lavender-top) tube - protected from light
Instructions
Pour serum or plasma into amber transport tube (preferred) or transport tube wrapped in aluminum foil to protect from light.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Refrigerated: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Serum separator tube (SST)
Methodology
Chromatography/Mass Spectrometry
Setup Schedule
A.M.
Clinical Significance
Vitamin B2 is involved in metabolism of fats, carbohydrates, and protein. The clinical manifestations of deficiency are non-specific. Clinical manifestations include mucocutaneous lesions of the mouth and skin, corneal vascularization, anemia, and personality changes.