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Epstein-Barr Virus DNA, Quantitative, Real-Time PCR
Test Code10186
CPT Codes
87799
Preferred Specimen
1 mL whole blood collected in an EDTA (lavender-top), ACD (yellow-top) , or PPT (white-top) tube or
1 mL plasma collected in an EDTA (lavender-top), ACD (yellow-top), or PPT (white-top) tube or
1 mL CSF in a sterile, plastic, leak-proof container or
1 mL serum
1 mL plasma collected in an EDTA (lavender-top), ACD (yellow-top), or PPT (white-top) tube or
1 mL CSF in a sterile, plastic, leak-proof container or
1 mL serum
Minimum Volume
0.5 mL
Other Acceptable Specimens
Bronchoalveolar lavage collected in a sterile, plastic, leak-proof container
Instructions
CSF: Collect in a sterile, plastic container and store refrigerated or frozen
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Heparin anticoagulant
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report available: 1 day
Clinical Significance
Monitoring EBV DNA levels by quantitative PCR in patients at risk of EBV-associated lymphoproliferative disorders may allow timely recognition of virus reactivation and permit installment of antiviral treatment.
