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Cholinesterase, Plasma (California Clients)
Test Code90368
CPT Codes
82480 <br>**This test is for California patients. For non-California patient testing, use 335.**
Preferred Specimen
1 mL plasma collected in a EDTA (lavender-top) tube
Minimum Volume
0.5 mL
Other Acceptable Specimens
Plasma collected in: PPT potassium EDTA (white-top) tube
Instructions
Draw an EDTA (lavender-top) tube of whole blood. Spin tube to separate plasma. Pour plasma into plastic aliquot tube and refrigerate until shipping. Do not send packed cells. Do not send one tube of whole blood.
Plasma cholinesterase results are not accurate if plasma sample is not separated from RBCs in a timely manner (within 1 hr). Hemolysis can lead to apparent increases in plasma cholinesterase activity, and could mask an enzyme deficiency.
Note: PPT potassium EDTA (white-top) tubes should be centrifuged within 24 hours.
Plasma cholinesterase results are not accurate if plasma sample is not separated from RBCs in a timely manner (within 1 hr). Hemolysis can lead to apparent increases in plasma cholinesterase activity, and could mask an enzyme deficiency.
Note: PPT potassium EDTA (white-top) tubes should be centrifuged within 24 hours.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 21 days
Refrigerated: 21 days
Frozen: 30 days
Refrigerated: 21 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis
Methodology
Kinetic Spectrophotometric (KS)
Setup Schedule
A.M. Sets up 5 days a week.
Clinical Significance
Approximately 1 in every 2500 individuals has inherited a defective enzyme or a deficiency of the enzyme (Pseudocholinesterase) that metabolizes succinylcholine (an anesthetic agent). With a "normal" dosage, these individuals have prolonged apnea. Such individuals are responsive at much smaller concentrations of this anesthetic agent than the general population. Low concentrations of Pseudocholinesterase are observed in individuals exposed to organophosphorous insecticides and in patients with hepatic dysfunction.
