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Direct Antiglobulin with Reflex Anti C3, Anti IgG
Test Code36668
CPT Codes
86880<br><strong>This test is not available for Rhode Island, Maryland, or New York patient testing.</strong>
Includes
If Direct Antiglobulin is positive, then Anti C3 and Anti IgG will be performed at an additional charge (CPT code(s): 86880 x2).
Preferred Specimen
5 mL whole blood collected in an EDTA (pink-top) tube
Minimum Volume
1 mL
Other Acceptable Specimens
Whole blood collected in: ACD (yellow-top) tube, EDTA (lavender-top) tube or Cord blood collected in a red top tube (no gel)
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: Unacceptable
Refrigerated: 72 hours
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Serum separator tube (SST) • Clotted specimen• Grossly icteric
Methodology
Agglutination
Setup Schedule
Sets up 5 days a week.
Clinical Significance
The DAT (Direct Coomb's test) is positive if red cells have been coated, in vivo, with immunoglobulin, complement, or both. A positive result can occur in immune-mediated red cell destruction, autoimmune hemolytic anemia, a transfusion reaction or in patients receiving certain drugs.
