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Influenza A and B Virus with Subtyping, Real-Time PCR
Test Code91335
CPT Codes
87502, 87503 (x3)
Includes
Influenza A RNA, QL PCR
Influenza B RNA, QL PCR
Influenza A H3
Influenza A H3N2V
2009 H1N1 Influenza RNA
Influenza B RNA, QL PCR
Influenza A H3
Influenza A H3N2V
2009 H1N1 Influenza RNA
Preferred Specimen
Nasopharyngeal or nasal swab in 3 mL M4 media or VCM medium (green-cap) tube or UTM equivalent
Minimum Volume
0.6 mL
Other Acceptable Specimens
Nasopharyngeal aspirate submitted in a sterile leak-proof container
Instructions
Swabs in VCM, M4 or UTM transport media: Use only sterile Dacron or Rayon swabs. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator stick off near the tip to permit tightening of the cap.
Nasopharyngeal aspirate: Submit in a sterile container with a leak-proof cap.
Nasopharyngeal aspirate: Submit in a sterile container with a leak-proof cap.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Calcium alginate swabs
Methodology
Real-Time Polymerase Chain Reaction • Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days
Clinical Significance
This test is used to determine the presence of Influenza A or B viral RNA in a patient's specimen, and to differentiate among possible Influenza A virus subtypes.
