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Herpesvirus 6 (HHV-6) DNA, Quantitative Real-Time PCR
Test Code19723
CPT Codes
87533
Preferred Specimen
1 mL whole blood collected in an EDTA (lavender-top) tube or
1 mL plasma collected in an EDTA (lavender-top) tube or
1 mL serum or
1 mL CSF collected in a sterile leak-proof container or
1 mL frozen bronchoalveolar lavage collected in a sterile leak-proof container or
1 mL frozen amniotic fluid collected in a sterile leak-proof container
1 mL plasma collected in an EDTA (lavender-top) tube or
1 mL serum or
1 mL CSF collected in a sterile leak-proof container or
1 mL frozen bronchoalveolar lavage collected in a sterile leak-proof container or
1 mL frozen amniotic fluid collected in a sterile leak-proof container
Minimum Volume
0.5 mL
Transport Temperature
Whole blood, plasma, serum, CSF: Refrigerated (cold packs)
Bronchoalvelar lavage, amniotic fluid: Frozen
Bronchoalvelar lavage, amniotic fluid: Frozen
Specimen Stability
Whole blood, plasma, serum, CSF
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Bronchoalveolar lavage, amniotic fluid
Room temperature: Unacceptable
Refrigerted: Unacceptable
Frozen: 30 days
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Bronchoalveolar lavage, amniotic fluid
Room temperature: Unacceptable
Refrigerted: Unacceptable
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Sodium heparin (green-top) tube • Lithium heparin (green-top) tube
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: 1-3 days
Clinical Significance
This test is used to determine the presence of HHV-6 DNA in patients' specimens. Organisms may be detected by PCR prior to detection by immunological methods. PCR provides more rapid results than other methods, including culture.