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Enterovirus RNA, Qualitative, Real-Time PCR
Test Code15082
CPT Codes
87498
Preferred Specimen
0.7 mL CSF collected in a sterile leak-proof container
Minimum Volume
0.3 mL CSF, plasma, serum or pericardial fluid • 0.5 g or 0.5 mL stool
Other Acceptable Specimens
Plasma collected in: EDTA (lavender-top) tube, PPT (white-top) tube, or ACD (yellow-top) tube • Serum • Rectal, throat, lesion or nasopharyngeal swab collected in a multi-microbe media, VCM medium (green-cap) tube, or equivalent (UTM) • 1 gram or 0.7 mL frozen stool collected in a sterile leak-proof container • Pericardial fluid collected in a sterile leak-proof container
Instructions
Specimens can be stored and transported either refrigerated or frozen. Avoid repeated freezing and thawing of specimens.
Transport Temperature
Stool: Frozen
All other samples: Refrigerated (cold packs)
All other samples: Refrigerated (cold packs)
Specimen Stability
All samples (except stool)
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Stool
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days
Methodology
Real-Time Polymerase Chain Reaction
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Sat; Report available: 1-2 days
Clinical Significance
Reverse Transcriptase-PCR (RT-PCR) is a highly sensitive method used to detect Enterovirus (EV) RNA from various clinical specimens. This assay targets a conserved region of the EV genome which allows detection of nearly the entire spectrum of human Enteroviruses, including the Coxsackieviruses, Polioviruses and the Echoviruses. It does not differentiate among the EV serotypes. The diagnosis of Enterovirus infection should not rely solely upon the result of a RT-PCR assay. A positive result should be considered in conjunction with clinical presentation and additional established clinical tests. A negative RT-PCR result indicates the absence of Enterovirus RNA in the sample tested and does not exclude the diagnosis of disease.
