West Nile Virus RNA, Qualitative Real-Time PCR

Test Code
17563X

CPT Codes
87798<br /> **This test is not available for New York patient testing**

Preferred Specimen
0.7 mL CSF in a sterile screw cap container


Minimum Volume
0.3 mL

Other Acceptable Specimens
Serum • Plasma collected in: EDTA (lavender-top) or ACD (yellow-top) tube

Instructions
Plasma: Collect blood in sterile tubes containing EDTA as anticoagulant or in Plasma Preparation Tube (PPT, preferred). Store collected whole at room temperature and separate plasma from cells within 2 hours of collection. Transfer plasma to sterile, plastic, screw-capped, aliquoted tubes. Do not clarify plasma by filtration or further centrifugation. If blood is collected in a PPT, centrifuge within 2 hours of collection but it is not necessary to transfer plasma to aliquot tube. Plasma can be shipped in PPT.
Serum: Collect blood in sterile tubes with no anticoagulants; serum separator tubes (SST) are recommended. Allow blood to clot at room temperature and separate serum from cells within 1 hour of collection. Transfer serum to sterile, plastic, screw-capped, aliquoted tubes.
CSF: Collect in a sterile, plastic container with a leak-proof cap.

Transport Temperature
Refrigerated (cold packs)

Specimen Stability
Room temperature: 48 hours
Refrigerated: 14 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Specimens containing heparin • Leaking, uncapped or broken containers • Plasma anticoagulated with heparin


Methodology
Real-Time Polymerase Chain Reaction

Setup Schedule

Set up: Mon-Sat; Report available: 1-2 days



Clinical Significance
This test is used to detect the presence of West Nile Virus
RNA in a patient's specimen. The use of real-time PCR to
assay for the presence of West Nile Virus RNA in clinical
specimens allows for rapid patient testing.



The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.