HIV-1 Resistance and Coreceptor Tropism, Proviral DNA

Test Code

94810

CPT Codes
87900, 87901, 87906 (x2)

Preferred Specimen

3 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

1.2 mL

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days

Methodology
Polymerase Chain Reaction (PCR) • Next Generation (ultra-deep) Sequencing

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Sets up 2 days a week.

Clinical Significance

This panel will assist clinicians to evaluate virologically suppressed patients for antiretroviral regimen modification when there is insufficient historical viral RNA resistance and viral tropism information. This test utilizes PCR and next generation (ultra-deep) sequencing of proviral DNA to detect drug resistance mutations in the HIV-1 protease, reverse transcriptase and integrase genes. As the CCR5 antagonist maraviroc may also be considered as a component of a new regimen, this panel includes the requisite tropism test performed on proviral DNA.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.