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Desmoglein Antibodies (1 and 3)
Test Code16033X
CPT Codes
83520 (x2)
Preferred Specimen
0.3 mL serum
Patient Preparation
Blood should be collected fasting or at least one hour after meals to avoid lipemic serum
Minimum Volume
0.1 mL
Instructions
Aseptically collect 5 to 8 mL of blood by approved venipuncture procedures.
Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. No anticoagulants, or preservatives should be added. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. These conditions may cause aberrant test results. Do not use heat-inactivated serum as this affects test results.
Allow the blood to clot at room temperature (20-25° C) and separate as soon as possible to limit hemolysis. No anticoagulants, or preservatives should be added. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. These conditions may cause aberrant test results. Do not use heat-inactivated serum as this affects test results.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
High levels of hemolysis • High levels of lipemia • Icterus or microbial growth
Methodology
Enzyme Linked Immunosorbent Immunoassay (ELISA)
Setup Schedule
A.M.
Clinical Significance
To detect the presence of auto antibody specific to Desmoglein 1 and/or 3 in a patients serum as an aid to diagnose type of pemphigus