HIV-1 Genotype and Coreceptor Tropism, Ultradeep Sequencing

Test Code

94015

CPT Codes
87900, 87901, 87906

Preferred Specimen

4 mL frozen plasma collected in an EDTA (lavender-top) tube

Minimum Volume

1.2 mL

Other Acceptable Specimens

Plasma collected in: PPT potassium EDTA (white-top) tube

Instructions

Do not freeze whole blood or any samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. If shipping frozen, transfer plasma to a leak-proof transport tube, then freeze prior to shipment. Follow manufacturer?s instructions for collection tube handling.

Transport Temperature

Frozen

Specimen Stability

Room temperature: 24 hours
Refrigerated: 5 days
Frozen: 30 days

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Gross hemolysis • Lipemia • Received room temperature • Heparinized plasma • Serum • Non-centrifuged PPT • Frozen PPT (in situ)

Methodology
Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) • Ultradeep Sequencing

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

See individual assays

Clinical Significance

Select antiretroviral drug regimen for treating HIV-1 patients by assessing reverse transcriptase and protease inhibitor resistance, together with CCR5 antagonist eligibility.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.