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Cardio IQ® Myeloperoxidase (MPO)
Test Code92814
CPT Codes
83876
Preferred Specimen
1 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
0.5 mL
Instructions
1. Draw and gently invert 8 to 10 times.
2. Centrifuge immediately for 10 minutes at 1300 RCF at room temperature.
3. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
Note: This ensures that the buffy coat and red cells remain undisturbed.
4. Aliquot plasma into labeled transport tube labeled as “EDTA plasma and cap tightly. Discard original tube.
5. Store transport tube refrigerated at 2-8°C until ready to ship.
Store plasma at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.
Erratic results can be observed if plasma is not centrifuged and separated from red cells immediately.
2. Centrifuge immediately for 10 minutes at 1300 RCF at room temperature.
3. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
Note: This ensures that the buffy coat and red cells remain undisturbed.
4. Aliquot plasma into labeled transport tube labeled as “EDTA plasma and cap tightly. Discard original tube.
5. Store transport tube refrigerated at 2-8°C until ready to ship.
Store plasma at 2-8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab, Inc. shipping box.
Erratic results can be observed if plasma is not centrifuged and separated from red cells immediately.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 8 days
Frozen: 6 months
Refrigerated: 8 days
Frozen: 6 months
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Samples other than EDTA plasma including PPT potassium EDTA (white-top) tube • Improper labeling • Samples not stored properly • Samples older than stability limits
Methodology
Turbidimetric Immunoassay
FDA Status
The test was developed and its performance characteristics determined by Cleveland HeartLab, Inc. It has not been cleared or approved by the U.S. FDA. The laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.
Setup Schedule
Sets up 5 days a week.
Report Available
Reports in 1 to 3 days.
Clinical Significance
Myeloperoxidase testing may be used for individuals with multiple risk factors for cardiovascular disease, or those with established disease.
