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PTH, Intact and Calcium
Test Code8837
CPT Codes
82310, 83970
Includes
The Intact PTH is the recommended initial assay for the differential diagnosis of calcium-related abnormalities
Preferred Specimen
1.5 mL plasma collected in EDTA (lavender-top) tube for the PTH, Intact and 1 mL serum for the Calcium
Minimum Volume
1 mL EDTA plasma for PTH, Intact • 0.5 mL serum for Calcium
Other Acceptable Specimens
2 mL (1 mL minimum) frozen serum for PTH, Intact
Instructions
Centrifuge and transfer plasma to a transport tube. Label as PLASMA for the PTH, Intact.
Transport Temperature
Frozen
Specimen Stability
EDTA plasma for PTH, Intact
Room temperature: 48 hours
Refrigerated: 72 hours
Frozen: 6 months
Serum for PTH, Intact
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 6 months
Serum for Calcium
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Room temperature: 48 hours
Refrigerated: 72 hours
Frozen: 6 months
Serum for PTH, Intact
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 6 months
Serum for Calcium
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Grossly lipemic • Plasma submitted in sodium heparin (green-top) tube or lithium heparin (green-top) tube
Methodology
PTH: Immunoassay (IA) • Calcium: Spectrophotometry (SP)
Setup Schedule
A.M. Sets up 5 days a week.
Clinical Significance
The assay is useful in making the diagnosis of primary hyperparathyroidism, secondary hyperparathyroidism, and a differential diagnosis of hypercalcemia. The assay helps in distinguishing hypercalcemia cause by either primary hyperparathyroidism or malignant disease.
