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HIV-1 RNA, Quantitative, Real-Time PCR with Reflex to Genotype (RTI, PI, Integrase)
Test Code91691
CPT Codes
87536
Includes
If HIV-1 RNA, Quantitative, Real-Time PCR viral load is >400 copies/mL, then HIV-1 Genotype and HIV-1 Integrase Genotype will be performed at an additional charge (CPT code(s): 87900, 87901, 87906).
Preferred Specimen
7 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
3.7 mL
Other Acceptable Specimens
Plasma collected in: PPT Potassium EDTA (white-top) tube
Instructions
Samples submitted in primary tube will be rejected. Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube.Follow manufacturer's instructions for collection tube handling.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 30 days
Refrigerated: 6 days
Frozen: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Gross hemolysis • Lipemia • Samples received in primary collection tube
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
Sets up 6 days a week.
Clinical Significance
Monitor viral load in HIV-1 patients undergoing antiretroviral therapy and automatically reflex to drug resistance testing for PR, RT and Integrase drugs in patients failing to achieve or maintain virologic suppression.