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HIV-1 RNA, Quantitative, Real-Time PCR
Test Code40085
CPT Codes
87536<br /> Reportable range: 20 to 10,000,000 HIV-1 RNA copies/mL (1.30 - 7.00 log copies/mL)
Preferred Specimen
3 mL plasma collected in an EDTA (lavender-top) or PPT (white-top) tube
Minimum Volume
1 mL
Instructions
Do not freeze whole blood or any samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation. Follow manufacturer’s instructions for collection tube handling.
Transport Temperature
Frozen
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 84 days
Refrigerated: 6 days
Frozen: 84 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received room temperature
Methodology
Real-Time Polymerase Chain Reaction
Setup Schedule
A.M. Sets up 6 days a week.
Report Available
Reports in 1 to 2 days.
Clinical Significance
This test is intended for use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV-1 infected patients. The test can be used to assess patient prognosis by measuring the baseline HIV-1 RNA level or to monitor the effects of antiretroviral therapy by measuring changes in EDTA plasma HIV-1 RNA levels during the course of antiretroviral treatment.
