HLA-B*15:02 Determination with Reflex to HLA-B High Resolution

Test Code

92746

CPT Codes
81374

Includes

If HLA-B*15:02 is Positive, HLA B-High Resolution will be performed at an additional charge (CPT code(s): 81380).

Preferred Specimen

14 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

5 mL

Other Acceptable Specimens

Whole blood collected in: ACD (yellow-top) tube, sodium heparin (green-top) or lithium heparin (green-top) tube

Note: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact BloodCenter of Wisconsin for collection, transport and stability instructions.

Instructions

Sample cannot be shipped on Saturday or the day before a holiday.

For Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.

For Pediatric patients: If requesting more than one panel for the HLA Transplant Testing, follow drawing instructions according to age as specified below. No more than what is specified by age is required.

Pediatric volume
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL

Transport Temperature

Room temperature

Specimen Stability

Whole blood
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swab
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received frozen • Heparinized or citrated tubes

Methodology
Polymerase Chain Reaction Amplification • Sequence Specific Oligonucleotide Probes

FDA Status
These tests were developed and their performance characteristics were determined by BloodCenter of Wisconsin. They have not been cleared by the FDA. However, this approval is not required.

Setup Schedule

Sets up 5 days a week.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.