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HLA-A29 Determination
Test Code16773
CPT Codes
81374
Preferred Specimen
14 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
5 mL
Other Acceptable Specimens
Whole blood collected in: ACD (yellow-top) tube or sodium heparin (green-top) or lithium heparin (green-top) tube • 4 buccal swabs
Note: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact BloodCenter of Wisconsin for collection, transport and stability instructions.
Note: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact BloodCenter of Wisconsin for collection, transport and stability instructions.
Instructions
Sample cannot be shipped on Saturday or the day before a holiday.
Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.
Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified below. No more than what is specified by age is required.
Pediatric volume
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL
Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.
Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified below. No more than what is specified by age is required.
Pediatric volume
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL
Transport Temperature
Room temperature
Specimen Stability
Whole blood
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swabs
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swabs
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Received frozen • Citrated tubes
Methodology
Polymerase Chain Reaction (PCR) • Sequence Specific Oligonucleotide Probes
FDA Status
These tests were developed and their performance characteristics were determined by BloodCenter of Wisconsin. They have not been cleared by the FDA. However, this approval is not required.
Setup Schedule
Set up: Mon-Sat; Report available: 5-7 days
