HLA-A,B Low Resolution Typing

Test Code

15757

CPT Codes
81373 (x2)

Preferred Specimen

14 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

5 mL whole blood

Other Acceptable Specimens

Whole blood collected in: ACD (yellow-top) tube, sodium heparin (green-top) tube or lithium heparin (green-top) tube • 4 buccal swabs
Note: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact BloodCenter of Wisconsin for collection, transport and stability instructions.

Instructions

Sample cannot be shipped on Saturday or the day before a holiday.

Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.

Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified below. No more than what is specified by age is required.

Pediatric volume
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL

Transport Temperature

Room temperature

Specimen Stability

Whole blood
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable

Buccal swabs
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Received frozen • Citrated tubes

Methodology
Polymerase Chain Reaction (PCR) • Sequence Specific Oligonucleotide Probes

Setup Schedule

Sets up 6 days a week.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.