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HLA-A*02:01 Determination
Test Code92044
CPT Codes
81380
Includes
Samples positive for A*0201 require HLA A-High resolution testing for an additional fee
Preferred Specimen
14 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
5 mL whole blood
Other Acceptable Specimens
Whole blood collected in: ACD (yellow-top) tube or sodium heparin (green-top) or lithium heparin (green-top) tube • 4 buccal swabs
Note: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact BloodCenter of Wisconsin for collection, transport and stability instructions.
Note: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact BloodCenter of Wisconsin for collection, transport and stability instructions.
Instructions
Sample cannot be shipped on Saturday or the day before a holiday.
Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.
Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified. No more than what is specified by age is required.
Pediatric volume
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL
Adult patients: If requesting more than one panel for HLA Transplant Testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.
Pediatric patients: If requesting more than one panel for HLA Transplant Testing, follow drawing instructions according to age as specified. No more than what is specified by age is required.
Pediatric volume
0-1 year: 3 mL
1-5 years: 5 mL
5-10 years: 7 mL
>10 years: 14 mL
Transport Temperature
Room temperature
Specimen Stability
Whole blood
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swabs
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Buccal swabs
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Hemolysis • Received room temperature >14 days • Received refrigerated • Received frozen
Methodology
Direct DNA Sequencing • Polymerase Chain Reaction (PCR)
FDA Status
These tests were developed and their performance characteristics were determined by BloodCenter of Wisconsin. They have not been cleared by the FDA. However, this approval is not required.
Setup Schedule
Sets up 7 days a week.
