HLA-C High Resolution

Test Code

17395

CPT Codes
81380

Preferred Specimen

14 mL whole blood collected in an EDTA (lavender-top) tube

Minimum Volume

5 mL

Other Acceptable Specimens

Whole blood collected in: ACD (yellow-top) tube, sodium heparin (green-top) or lithium heparin (green-top) tube • 4 buccal swabs

Note: For cord blood, bone marrow and peripheral blood leukocyte specimen submissions, please contact BloodCenter of Wisconsin for collection, transport and stability instructions.

Instructions

Sample cannot be shipped on Saturday or the day before a holiday.

For adult patients:
If requesting more than one panel for HLA transplant testing, no more than 14 mL whole blood collected in an EDTA (lavender-top) tube is required.

For pediatric patients:
If requesting more than one panel for HLA transplant testing, follow drawing instructions according to age as specified. No more than what is specified by age is requred.

Pediatric volume:

0-1 year	3 mL
1-5 years	5 mL
5-10 years	7 mL
>10 years	14 mL

Transport Temperature

Room temperature

Specimen Stability

Whole blood
Room temperature: 14 days
Refrigerated: Unacceptable
Frozen: Unacceptable

Buccal swabs
Room temperature: 30 days
Refrigerated: Unacceptable
Frozen: Unacceptable

Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)

Hemolysis • Received room temperature >14 days • Received refrigerated • Received frozen

Methodology
Direct DNA Sequencing • Polymerase Chain Reaction (PCR)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

Sets up 6 days a week.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.