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Fecal Leukocyte Stain
Test Code3930
CPT Codes
89055
Preferred Specimen
10 grams or 10 mL stool collected in Zn-PVA or Total-Fix® transport vial
Patient Preparation
Patients should refrain from ingesting Barium for 7 days before specimen collection
Minimum Volume
5 gm or 5 mL
Instructions
1. The specimen must be passed into a clean dry container and must not be contaminated with urine or water. If a toilet is used, the water supply may be cut off and the bowl drained.
2. Add stool to bring the liquid level to the "Fill to here" line on the Para-Pak Zn PVA or Total-Fix® vial. Mix contents thoroughly until homogeneous.
2. Add stool to bring the liquid level to the "Fill to here" line on the Para-Pak Zn PVA or Total-Fix® vial. Mix contents thoroughly until homogeneous.
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 6 months
Refrigerated: Not recommended
Frozen: Unacceptable
Refrigerated: Not recommended
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Stool in Cary-Blair transport medium • Received frozen • Specimens containing Barium or received in preservatives other than Zn-PVA or Total-Fix® • Unpreserved stool
Methodology
Microscopy
Setup Schedule
A.M.
Limitations
In 10-15% of patients with invasic bacterial infection, their fecal stool will not reveal leukocytes. Fecal leukocytes may be present in idiopathic inflammatory bowel disease.
Clinical Significance
The presence of leukocytes is an indicator of inflammation. Generally, inflammation is a product of bacteria-host interaction.
