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Microsporidia Exam
Test Code3562
CPT Codes
87207
Includes
Smear and Concentration (if performed)
Preferred Specimen
5 grams or 5 mL stool preserved in 10% formalin or Total-Fix® transport vial
Minimum Volume
2 mL or 2 grams
Other Acceptable Specimens
Duodenal aspirate • Conjunctival/corneal scrapings: slide 10% formalin fixed or slide methanol fixed • 5 mL CSF, BAL/nasal secretions, urine sediments in a sterile screw-cap container • CSF, BAL/nasal secretions, urine sediments: slide 10% formalin fixed or slide methanol fixed
Instructions
1. Stool and duodenal aspirates fixed in 10% formalin or Total-Fix®
2. Urine sediments, CSF, BAL/Nasal secretions: preferred in sterile container, acceptable on microscope slide, methanol (or 10% formalin).
3. Conjunctival/corneal scrapings, preferred on microscope slide, methanol (or 10% formalin).
Ship preserved specimens at room temperature (preferred).
2. Urine sediments, CSF, BAL/Nasal secretions: preferred in sterile container, acceptable on microscope slide, methanol (or 10% formalin).
3. Conjunctival/corneal scrapings, preferred on microscope slide, methanol (or 10% formalin).
Ship preserved specimens at room temperature (preferred).
Transport Temperature
Preserved or fixed: Room temperature
Raw specimens: Refrigerated (cold packs)
Raw specimens: Refrigerated (cold packs)
Specimen Stability
Preserved or fixed
Room temperature: 30 days
Refrigerated: 30 days
Frozen: Unacceptable
Raw specimens
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Room temperature: 30 days
Refrigerated: 30 days
Frozen: Unacceptable
Raw specimens
Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: Unacceptable
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Unpreserved stool • Stool in Cary Blair or Polyvinyl Alcohol (PVA) • Received frozen
Methodology
Microscopy
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Mon-Fri; Report available: 1-3 days
Clinical Significance
Microsporidia infection was first recognized as a cause of chronic diarrhea in patients infected with HIV. Microsporidia may also cause pneumonia, acute bilateral keratoconjunctivitis, and infection of the biliary and pancreatic ducts.
