FISH, High-Grade Lymphoma Panel

Test Code

91606

CPT Codes
88271 (x6), 88275 (x3)

Preferred Specimen

Formalin-fixed, paraffin-embedded tissue

Minimum Volume

1 mL bone marrow
5x5 mm tissue

Other Acceptable Specimens

Bone marrow collected in: Sodium heparin (green-top) tube, sodium heparin (royal blue-top) tube, sodium heparin lead-free (tan-top) tube • 5x5 mm lymph node/tumor collected in paraffin block

Instructions

Bone marrow: 1-3 mL in transport medium (preferred) or sodium heparin (green-top, dark/royal blue-top or tan-top) tubes are acceptable containers for this test.
Lymph node/tumor: 5x5 mm fresh tumor biopsy in a transport medium.
Paraffin block: Formalin fixed paraffin embedded tissue. Transport media available upon request. Ship at room temperature. Do not Freeze.

Transport Temperature

Room temperature

Specimen Stability

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Methodology
Fluorescence in situ Hybridization (FISH)

FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Setup Schedule

A.M. Sets up 7 days a week.

Clinical Significance

High-grade B-cell lymphoma can be difficult to classify if histologic and immunophenotypic features are not entirely typical. Large B-cell lymphoma with monomorphic appearance or very high mitotic rate and Burkitt lymphoma cases that have atypical immunophenotypic or morphologic appearances present diagnostic difficulties. The FISH panel for MYC (8q24), t(14;18) (IGH-BCL2) and BCL6 (3q27) translocations, provides additional diagnostic information that may help in final classification and therapy selection in high-grade B-cell lymphomas as well as to assess for "double-hit" lymphomas and "triple-hit" lymphomas.

The CPT Codes provided in this document are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payor being billed. Any Profile/panel component may be ordered separately. Reflex tests are performed at an additional charge.