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T-cell Receptor (TCR) Gamma Gene Rearrangement, PCR
Test Code90509
CPT Codes
81342, 84999 (HCPCS: G0452)
Includes
Pathologist Interpretation
Preferred Specimen
5 mL whole blood or 3 mL bone marrow aspirate collected in an EDTA (lavender-top) tube or
8 unstained charged (+) slides
8 unstained charged (+) slides
Minimum Volume
3 mL whole blood • 1 mL bone marrow aspirate • 4 unstained charged (+) slides
Other Acceptable Specimens
Formalin fixed paraffin embedded tissue • Fresh tissue in a sterile transport tube • Whole blood or bone marrow collected in a sodium heparin (green-top) tube
Instructions
8 unstained sections on glass slides labeled with surgical case number (pathology report required, indicate if fixative is not formalin). Please include pathology report for frozen tissue and FFPE cases (partial/preliminary reports acceptable).
Needle washings (acceptable): In alcohol based fixative (e.g. CytoLyt®) are acceptable.
Do not reject specimens, send to laboratory for screening.
Needle washings (acceptable): In alcohol based fixative (e.g. CytoLyt®) are acceptable.
Do not reject specimens, send to laboratory for screening.
Transport Temperature
All other specimens: Room temperature
Fresh Tissue: Frozen
Fresh Tissue: Frozen
Specimen Stability
Whole blood and bone marrow aspirate
Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable
FFPE tissue/slides
Room temperature: 5 years
Refrigerated: 5 years
Frozen: Unacceptable
Fresh tissue
Room temperature: See Collection Instructions
Refrigerated: See Collection Instructions
Frozen -20° C: See Collection Instructions
Frozen -70° C: 1 year
Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable
FFPE tissue/slides
Room temperature: 5 years
Refrigerated: 5 years
Frozen: Unacceptable
Fresh tissue
Room temperature: See Collection Instructions
Refrigerated: See Collection Instructions
Frozen -20° C: See Collection Instructions
Frozen -70° C: 1 year
Methodology
Polymerase Chain Reaction (PCR) • Fragment Analysis
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Setup Schedule
Set up: Daily; Report Available: 5 days
Clinical Significance
This assay which interrogates the T-cell receptor gamma locus (TCRG) by a PCR method based on the BIOMED-2 consensus, is used for establishing clonality of T-cell lymphoid neoplasms and identification of minimal residual disease or early recurrence in patients with a previous diagnosis of a T-cell neoplasm. It can be used in association with T-cell receptor beta (TCBR) PCR assay (Test code 91446 or TCRG/TCRB combo test code 91445), since false-negative results can occur in up to 5-10% of T-cell malignancies when testing TCRG PCR only.