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Influenza A and B RNA, Qualitative RT-PCR, Conjunctiva with Reflex to H5
Test Code18203
CPT Codes
87502<br><strong> This test is not available for New York patient testing.</strong>
Includes
If Influenza A RNA, PCR is Detected, then Influenza A Virus RNA, H5 Subtyping, Qualitative Real-Time PCR will be performed at an additional charge (CPT code(s): 87503).
Preferred Specimen
1 or 2 conjunctiva swabs submitted in a 3 mL VCM (green-cap) or equivalent (UTM) tube
Minimum Volume
0.6 mL
Instructions
Each Influenza type A/B RNA with reflex to H5 specimen should be accompanied by its own separate requisition and transported in its own sealed bag. If swabbing both eyes, it is acceptable to place both swabs into one vial of VCM or equivalent.
This test is not approved for observed-self collection at Quest Patient Service Centers.
This test is not approved for observed-self collection at Quest Patient Service Centers.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Calcium alginate swabs • Wooden shafted swabs • Nasal specimens
Methodology
Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Set up: Daily; Report available: 1-2 days
Clinical Significance
This test is for detection of Influenza A virus, with reflex to H5 typing. The H5 reflex is a qualitative molecular diagnostic test performed on Influenza A positive samples to identify Influenza A H5 virus, including H5N1, commonly known as Avian Influenza. The reflex test does not subtype for seasonal influenza viruses. This test is intended to be performed on conjunctiva specimens collected from individuals suspected of infection with Influenza A H5, or who meet the Centers for Disease Control and Prevention (CDC) clinical and/or epidemiological criteria for Influenza A H5N1 testing. For details visit: https://www.cdc.gov/bird-flu/hcp/clinicians-evaluating-patients.
Laboratory test results should always be considered in the context of clinical observations and epidemiological data in making a final diagnosis and patient management decisions.
Laboratory test results should always be considered in the context of clinical observations and epidemiological data in making a final diagnosis and patient management decisions.

