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Influenza A and B RNA, Qualitative RT-PCR, Respiratory with Reflex to H5
Test Code18201
CPT Codes
87502 <br><strong>This test is not available for New York patient testing.</strong>
Includes
If Influenza A RNA, PCR is Detected, then Influenza A Virus RNA, H5 Subtyping, Qualitative Real-Time PCR will be performed at an additional charge (CPT code(s): 87503).
Preferred Specimen
Nasopharyngeal swab or anterior nares or oropharyngeal swab submitted in a 3 mL VCM (green-cap) or equivalent (UTM) tube
Minimum Volume
0.6 mL VCM or UTM • 1 mL bronchial lavage/wash
Other Acceptable Specimens
1 mL bronchial lavage/wash collected in a sterile leak-proof container
Instructions
Each Influenza type A/B RNA with reflex to H5 specimen should be accompanied by its own separate requisition and transported in its own sealed bag.
This test is not approved for observed-self collection at Quest Patient Service Centers.
This test is not approved for observed-self collection at Quest Patient Service Centers.
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Room temperature: Unacceptable
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Refrigerated: 4 days
Frozen -20° C: Unacceptable
Frozen -70° C: 30 days
Reject Criteria (Eg, hemolysis? Lipemia? Thaw/Other?)
Calcium alginate swabs • Wooden shafted swabs • Nasal specimens
Methodology
Real-Time Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)
Setup Schedule
Setup: Daily; Report available: 1-2 days
Clinical Significance
This screening test reflexes to the Influenza A (H5) Virus RNA, Qualitative Real-Time PCR test when influenza A is detected. The H5 reflex is a qualitative multi-target molecular diagnostic test that aids in the detection of the Influenza A H5 virus, including H5N1, commonly known as Avian Influenza. The reflex test does not detect Influenza B or other respiratory viurses. This test is intended to be performed on respiratory specimens collected from individuals suspected of infection with Influenza A H5, or who meet the Centers for Disease Control and Prevention (CDC) clinical and/or epidemiological criteria for Influenza A H5N1 testing. For details visit: https://www.cdc.gov/bird-flu/hcp/clinicians-evaluating-patients.
Laboratory test results should always be considered in the context of clinical observations and epidemiological data in making a final diagnosis and patient management decisions.
Laboratory test results should always be considered in the context of clinical observations and epidemiological data in making a final diagnosis and patient management decisions.
